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KMID : 0380020090240010065
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Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 1 p.65 ~ p.69
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Effect of Dissolved Oxygen on the Production of Epothilone in Bioreactor Cultures Sorangium cellulosum
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Park Su-Jeong
Han Se-Jong
Kim Byung-Woo
Sim Sang-Jun
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Abstract
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The biological production of a potent anticancer agent, epothilone, by Sorangium cellulosum was carried out using flask and fermentor cultures. Soluble starch was selected as the main carbon source and the concentrations of lactose and yeast extract were optimized at 4 and 0 g/L, respectively, when using the flask cultures. In the fermentor cultures, the cells were cultivated at a high DO level of more than 80% of air saturation in the growth stage and then the DO level was controlled at about 50, 20 or 1-2% when the carbon source was exhausted. The epothilone production increased with decreasing DO level after the exhaustion of the carbon source, and the maximum concentration of epothilone was 5.4 mg/L. It was found that the DO level had significant regulation effects on the epothilone production.
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KEYWORD
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Dissolved Oxygen (DO), Epothilone, Secondary metabolite, Sorangium cellulosum
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